Journal: Applied and Environmental Microbiology

Article Title: The insertion of the inverted repeat of an insertion sequence (IS) element from Lacticaseibacillus rhamnosus changes the host range and stability of pGK12, a shuttle vector for lactic acid bacteria

doi: 10.1128/aem.01908-24

Figure Lengend Snippet: Plasmids used in this study

Article Snippet: The segregational stability of pGK12, pTRK829, and pTRK830 was tested in six different LAB strains (LGG, L. casei ATCC 393, L. paracasei ATCC 25598, L. paracasei ATCC 334, L. gasseri ATCC 33323, and L. johnsonii ATCC 11506) under non-selective conditions.

Techniques: Plasmid Preparation, Expressing, Clone Assay

Journal:

Article Title: In Vitro Adhesion Specificity of Indigenous Lactobacilli within the Avian Intestinal Tract

doi: 10.1128/AEM.68.10.5155-5159.2002

Figure Lengend Snippet: Adherence of chicken-associated lactobacilli isolates to immobilized ileal mucus. Adherence to mucus preparations is shown by filled symbols, and the adherence to type IV collagen is shown by open symbols. (A) Adherence of L. crispatus strains ST1 (•), A33 (▾), 134mi (▪), and ATCC 33820 (⧫). (B) Adherence of L. reuteri strains CT7 (•) and ATCC 53609 (▾). (C) Adherence of L. gasseri strains CT5 (•) and ATCC 33323 (▾). (D) For comparison, the adhesion by strains 1063 of L. reuteri (•) and ATCC 393 of L. casei (▾) are shown.

Article Snippet: The strains ATCC 33323 of L. gasseri, ATCC 53609 of L. reuteri , and ATCC 393 of L. casei showed only poor adhesiveness to the chicken tissue (data not shown). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1. caption a7 Adherence of L. crispatus strains ST1 (AI through FI) and ATCC 33820 (GI) to regions of the chicken alimentary tract.

Techniques: Comparison

Journal:

Article Title: Supplementation of the Diet with High-Viscosity Beta-Glucan Results in Enrichment for Lactobacilli in the Rat Cecum

doi: 10.1128/AEM.72.3.1925-1931.2006

Figure Lengend Snippet: Utilization by lactobacilli of β-glucan hydrolysate enriched for DP3 and DP4 oligosaccharides (72 h of anaerobic incubation at 37°C) a

Article Snippet: The utilization of oligosaccharides in β-glucan hydrolysates was measured by the culture of strains of lactobacilli ( L. acidophilus ATCC 4356 T , Lactobacillus crispatus ATCC 33820 T , Lactobacillus gasseri ATCC 33323 T , Lactobacillus johnsonii ATCC 33200 T , Lactobacillus hamsteri ATCC 43851 T , and Lactobacillus ruminis strains ATCC 27780 T , ATCC 27781, and ATCC 27782) in peptone-yeast extract medium supplemented with vitamin K-hemin, Tween 80, and l -cysteine ( 21 ) and containing a 2% (wt/vol) concentration of hydrolysate.

Techniques: Incubation

Journal:

Article Title: Identification and Cloning of gusA , Encoding a New ?-Glucuronidase from Lactobacillus gasseri ADH †

doi: 10.1128/AEM.67.3.1253-1261.2001

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Further confirmation of one clone, designated pTRK666, was obtained by subcloning its 4.5-kb insert as an Eco RI- Pst I fragment into the gram-positive shuttle vector, pTRK563, and introducting the resulting plasmid (pTRK668) into L. gasseri ATCC 33323 by electroporation.

Techniques: Plasmid Preparation, Expressing, Clone Assay